Hossein Piri; Benyamin Alimohammadi; Fatemeh Saeedi; Firoozeh Naderi; Hasan Azhdari-Zarmehri
Volume 23, Issue 1 , May and June 2016, , Pages 151-160
Abstract
Background and Objectives: About half to one percent of the world population suffer from epilepsy.Seizure is the most common symptoms of epilepsy due to abnormal electrical discharge of neurons in the brain. Due to side effects of chemical drugs, herbal plants are widely used to control seizures. In ...
Read More
Background and Objectives: About half to one percent of the world population suffer from epilepsy.Seizure is the most common symptoms of epilepsy due to abnormal electrical discharge of neurons in the brain. Due to side effects of chemical drugs, herbal plants are widely used to control seizures. In this study, Anticonvulsant activity of hydro-alcoholicextract of (Ziziphoratenuior L.) was investigated in mice. Materials and Methods: In this study, 40 male mice (25-30 g) were randomly selected and divided into five groups of 8, that included a control group (receiving saline and PTZ) and 4 treatment groups (receiving the extract doses of 150, 300, 600, 900 mg / kg). 30 minutes after IP administration with saline (in control group) and different doses of extract (in treatment groups), PTZ (80 mg/kg) were injectedAnd were transferred to a special cage immediately and convulsive behaviors were recorded by a camera during the 20 minutes. Then, the different phases of seizure were evaluated. Results: Extract injection in all doses by dose- dependently delayed the onset time of clonic, tonic and tonic-clonic seizures, but doses of 600 and 900 mg/kg had showed the greatest effect on onset time. The duration time of tonic, clonic and tonic-clonic seizures in animals that received extract also reduced. Extract injection also dose-dependently decreased the rate of mortality and the number of suddenly jumping during seizures, these effects in doses of 600 and 900 mg/kg was significant. Conclusion: Our study showed that, the hydro-alcoholicextract of (Ziziphoratenuior L.) had an appropriate anticonvulsant activity and it seems the future studies will be necessary to separate its ingredients and understand its mechanism of action.
Benyamin Alimohammadi; Alireza Moslem; Hassan Azhdari Zarmehri; Homan Kamranian
Volume 23, Issue 2 , March and April 2016, , Pages 204-213
Abstract
Background and Objectives: Nowadays the use of herbal plants due to their low complications attracts the mind of many scientists. The aim of this study is the assessment of the Naloxone as an antagonist of opioids on seizure duration time induced by PTZ and determine the anticonvulsant mechanism of hydro ...
Read More
Background and Objectives: Nowadays the use of herbal plants due to their low complications attracts the mind of many scientists. The aim of this study is the assessment of the Naloxone as an antagonist of opioids on seizure duration time induced by PTZ and determine the anticonvulsant mechanism of hydro alcoholic extract of Scrophularia striata Boiss. Materials & Methods: In the present study, 40 male mice were randomly divided into five groups of eight; The control group (receiving PTZ 80 mg/kg, i.p.), Two treatment groups (Scrophularia striata 600 and 900 mg/kg, i.p.) and two experimental groups (Scrophularia striata 600 and 900 mg/kg i.p plus Naloxone 5 mg/kg, i.p., 5 minutes before extract injection). With injection of PTZ, convulsive behaviors in mice during 20 minutes were recorded by camera and the various stages of seizures were evaluated. Statistical data were analyzed using with the tests of one way Variance and Tukey in SPSS 16. PResults: Statistical analysis showed that Naloxone injection with extract administration have slightly and not significantly decreased the latency time to tonic and clonic seizure in comparison with the control group. Latency time to tonic-colonic has been increased in extract only group and extract associated Naloxone group. With injection of the extract alone total seizure time has decreased but in extract associated Naloxone group this time has increased that this increasing in dose of 600 mg/kg is significant in comparison with control group (P<0.01). Data showed that extract injection lonely and associated with Naloxone have increased the duration time of tonic seizure, duration time of tonic-colonic with extract injection was increased but this time in Naloxone group has been slightly decreased. Conclusion: Our study indicated that Naloxone as an antagonist can inhibit the anticonvulsant activity of hydro alcoholic extract of Scrophularia striata Boiss.